abstract：BACKGROUND:Commercial immunoassays for testosterone (Te) may give inaccurate results for samples from women and children, leading to misdiagnosis and inappropriate treatment. We developed a sensitive and specific tandem mass spectrometric assay for measurement of Te at the concentrations encountered in women and childr...

journal_title：Clinical chemistry

authors： Kushnir MM,Rockwood AL,Roberts WL,Pattison EG,Bunker AM,Fitzgerald RL,Meikle AW

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:Rapid detection of biological threat agents is critical for timely therapeutic administration. Fluorogenic PCR provides a rapid, sensitive, and specific tool for molecular identification of these agents. We compared the performance of assays for 7 biological threat agents on the Idaho Technology, Inc. R.A.P....

journal_title：Clinical chemistry

authors： Christensen DR,Hartman LJ,Loveless BM,Frye MS,Shipley MA,Bridge DL,Richards MJ,Kaplan RS,Garrison J,Baldwin CD,Kulesh DA,Norwood DA

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:The lead mobilization test reflects the mobilizable and likely toxicologically active fraction of the lead body burden. We propose a safe and convenient protocol for this test, to assess concomitant copper and zinc excretion and to determine the size of the chelatable lead pool in nonoccupationally exposed a...

journal_title：Clinical chemistry

authors： Hoet P,Buchet JP,Decerf L,Lavalleye B,Haufroid V,Lison D

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:Urinary cross-linked N-telopeptide of type I collagen (NTX) is a reliable bone resorption marker in patients with metabolic bone disease. We assessed a clinically available serum NTX assay suitable for anuric patients on hemodialysis (HD). METHODS:Serum concentrations of NTX, C-terminal telopeptide of type ...

journal_title：Clinical chemistry

authors： Maeno Y,Inaba M,Okuno S,Yamakawa T,Ishimura E,Nishizawa Y

更新日期：2005-12-01 00:00:00

abstract：BACKGROUND:Published literature was systematically reviewed to determine the diagnostic accuracy of new protein markers of acute coronary syndromes (ACS) in symptomatic outpatients at low risk of ACS and related complications comparable to patients evaluated in emergency department chest pain units. METHODS:Studies we...

journal_title：Clinical chemistry

authors： Mitchell AM,Brown MD,Menown IB,Kline JA

更新日期：2005-11-01 00:00:00

abstract：BACKGROUND:The susceptibility of plasma lipids to oxidation is thought to be a factor contributing to atherogenic risk. Various groups have studied the in vitro oxidizability of isolated LDL and examined the effects of conventional antioxidants. The drawbacks associated with the isolation of LDL for evaluation of in vi...

journal_title：Clinical chemistry

authors： Atkin MA,Gasper A,Ullegaddi R,Powers HJ

更新日期：2005-11-01 00:00:00

abstract：BACKGROUND:Yersinia pestis, the causative agent of the zoonotic infection plague, is a major concern as a potential bioweapon. Current real-time PCR assays used for Y. pestis detection are based on plasmid targets, some of which may generate false-positive results. METHODS:Using the yp48 gene of Y. pestis, we designed...

journal_title：Clinical chemistry

authors： Chase CJ,Ulrich MP,Wasieloski LP Jr,Kondig JP,Garrison J,Lindler LE,Kulesh DA

更新日期：2005-10-01 00:00:00

abstract：BACKGROUND:Quantification of 5-hydroxyindole-3-acetic acid (5-HIAA) in urine is useful in diagnosing and monitoring of patients with carcinoid tumors and in the study of serotonin (5-hydroxytryptamine) metabolism in various disorders. We describe an automated method that incorporates on-line solid-phase extraction (SPE...

journal_title：Clinical chemistry

authors： Mulder EJ,Oosterloo-Duinkerken A,Anderson GM,De Vries EG,Minderaa RB,Kema IP

更新日期：2005-09-01 00:00:00

abstract：BACKGROUND:It has been postulated that changes in S-adenosylhomocysteine (AdoHcy), a potent inhibitor of transmethylation, provide a mechanism by which increased homocysteine causes its detrimental effects. We aimed to develop a rapid and sensitive method to measure AdoHcy and its precursor S-adenosylmethionine (AdoMet...

journal_title：Clinical chemistry

authors： Gellekink H,van Oppenraaij-Emmerzaal D,van Rooij A,Struys EA,den Heijer M,Blom HJ

更新日期：2005-08-01 00:00:00

abstract：BACKGROUND:Tacrolimus is metabolized predominantly to 13-O-demethyltacrolimus in the liver and intestine by cytochrome P450 3A (CYP3A). Patients with high concentrations of CYP3A5, a CYP3A isoenzyme polymorphically produced in these organs, require higher doses of tacrolimus, but the exact mechanism of this association...

journal_title：Clinical chemistry

authors： Kamdem LK,Streit F,Zanger UM,Brockmöller J,Oellerich M,Armstrong VW,Wojnowski L

更新日期：2005-08-01 00:00:00

abstract：BACKGROUND:The aim of our present study was to establish new reference intervals for thyrotropin (TSH) and thyroid hormones based on National Academy of Clinical Biochemistry (NACB) criteria and regular thyroid ultrasonography. We also assessed the effect of potentially confounding factors to modulate the limits of the...

journal_title：Clinical chemistry

authors： Kratzsch J,Fiedler GM,Leichtle A,Brügel M,Buchbinder S,Otto L,Sabri O,Matthes G,Thiery J

更新日期：2005-08-01 00:00:00

abstract：BACKGROUND:Age-adjusted morbidity and mortality rates from coronary heart disease (CHD) are higher in men than in women. Androgens are suspected to be responsible for the male disadvantage. The genomic effect of androgens is mediated by the androgen receptor (AR), which has a polymorphic CAG repeat in exon 1. The numbe...

journal_title：Clinical chemistry

authors： Hersberger M,Muntwyler J,Funke H,Marti-Jaun J,Schulte H,Assmann G,Lüscher TF,von Eckardstein A

更新日期：2005-07-01 00:00:00

abstract：BACKGROUND:Epidemiologists have observed that blood donation is associated with decreased risk of type 2 diabetes and cardiovascular disease. METHODS:We investigated the relationship between iron stores and insulin sensitivity, after controlling for known confounding factors, and compared insulin sensitivity between b...

journal_title：Clinical chemistry

authors： Fernández-Real JM,López-Bermejo A,Ricart W

更新日期：2005-07-01 00:00:00

abstract：:Values for insulin-mediated glucose disposal vary continuously throughout a population of apparently healthy individuals, with at least a sixfold variation between the most insulin sensitive and most insulin resistant of these individuals. The more insulin resistant a person, the more insulin must be secreted to preve...

journal_title：Clinical chemistry

authors： Reaven GM

更新日期：2005-06-01 00:00:00

abstract：BACKGROUND:Detection of melanoma cells in circulation may be important in assessing tumor progression. The objective of this study was to develop a specific, reliable multimarker quantitative real-time reverse transcription-PCR (qRT) assay for detecting melanoma cells in patients' blood. METHODS:We developed qRT assay...

journal_title：Clinical chemistry

authors： Koyanagi K,Kuo C,Nakagawa T,Mori T,Ueno H,Lorico AR Jr,Wang HJ,Hseuh E,O'Day SJ,Hoon DS

更新日期：2005-06-01 00:00:00

abstract：BACKGROUND:High circulating concentrations of N-terminal fragments of A- and B-type natriuretic peptides (NT-proANP and NT-proBNP) identify patients with impaired cardiac function. ProANP-derived peptides are particularly sensitive to increased preload of the heart and proBNP-derived peptides to increased afterload; th...

journal_title：Clinical chemistry

authors： Ala-Kopsala M,Ruskoaho H,Leppäluoto J,Seres L,Skoumal R,Toth M,Horkay F,Vuolteenaho O

更新日期：2005-04-01 00:00:00

abstract：BACKGROUND:Unpredictable efficacy and toxicity are hallmarks of most anticancer therapies. Predictive markers are factors that are associated with response or resistance to a particular therapy. METHODS:The English literature relating to predictive markers in oncology was reviewed. Particular attention was paid to met...

journal_title：Clinical chemistry

authors： Duffy MJ

更新日期：2005-03-01 00:00:00

abstract：BACKGROUND:We explored the utility of multiplexed temperature gradient capillary electrophoresis (TGCE) as a screening tool for identifying genetic changes in the human mitochondrial genome. We examined changes in mitochondrial DNA (mtDNA) in nonmelanoma skin cancers (NMSCs), using TGCE to resolve genetic differences c...

journal_title：Clinical chemistry

authors： Girald-Rosa W,Vleugels RA,Musiek AC,Sligh JE

更新日期：2005-02-01 00:00:00

abstract：BACKGROUND:The ratio of plasma aldosterone concentration to plasma renin activity (PRA) is considered the screening test of choice for primary aldosteronism. Uncertainty exists, however, regarding its diagnostic accuracy and the effects of antihypertensive drugs and dietary sodium balance on test characteristics. METH...

journal_title：Clinical chemistry

authors： Schwartz GL,Turner ST

更新日期：2005-02-01 00:00:00

abstract：BACKGROUND:The recent development of nonradioactive automated assays for serum parathyroid hormone (PTH) and 25-hydroxyvitamin D (25OHD) has made measurement of these two hormones possible in many laboratories. In this study, we compared two new assays for PTH and 25OHD adapted on an automated analyzer, the LIAISON, wi...

journal_title：Clinical chemistry

authors： Souberbielle JC,Fayol V,Sault C,Lawson-Body E,Kahan A,Cormier C

更新日期：2005-02-01 00:00:00

abstract：BACKGROUND:The applicability of microarray-based transcriptome massive analysis is often limited by the need for large amounts of high-quality RNA. RNA arbitrarily primed PCR (RAP-PCR) is an unbiased fingerprinting PCR technique that reduces both the amount of initial material needed and the complexity of the transcrip...

journal_title：Clinical chemistry

authors： Grau M,Solé X,Obrador A,Tarafa G,Vendrell E,Valls J,Moreno V,Peinado MA,Capellá G

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND:Non-(1-84) parathyroid hormone (PTH) fragments are large C-terminal fragments of PTH with a partially preserved N-terminal structure. They differ from other C-terminal PTH fragments, which do not have an N-terminal structure and do not react in intact PTH assays. We aimed to identify the minimal N-terminal s...

journal_title：Clinical chemistry

authors： D'Amour P,Brossard JH,Räkel A,Rousseau L,Albert C,Cantor T

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND:Oxidation of lipids in lipoproteins and cells may initiate and enhance the early development of cardiovascular disease. METHOD AND RESULTS:We assayed F(2)-isoprostanes, oxidation products of arachidonic acid, by gas chromatography-mass spectrometry in a biracial cohort of 2850 young healthy adult men and wo...

journal_title：Clinical chemistry

authors： Gross M,Steffes M,Jacobs DR Jr,Yu X,Lewis L,Lewis CE,Loria CM

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND:Atherosclerosis is a complex, chronic disease that usually arises from the converging action of several pathogenic processes, including hypertension, hyperlipidemia, obesity, and the accumulation of oxidized LDL. Platelet-activating factor acetylhydrolase (PAF-AH) is a LDL- and HDL-bound enzyme that hydrolyz...

journal_title：Clinical chemistry

authors： Campo S,Sardo MA,Bitto A,Bonaiuto A,Trimarchi G,Bonaiuto M,Castaldo M,Saitta C,Cristadoro S,Saitta A

更新日期：2004-11-01 00:00:00

abstract：BACKGROUND:A sensitive gas chromatography-negative ion chemical ionization mass spectrometry (GC/MS-NICI) method was developed and validated for the measurement of Delta(9)-tetrahydrocannabinol (THC) in human sweat patches. METHODS:THC-d(0) and THC-d(3) were added to worn blank sweat patches (PharmChek; PharmChem Inco...

journal_title：Clinical chemistry

authors： Saito T,Wtsadik A,Scheidweiler KB,Fortner N,Takeichi S,Huestis MA

更新日期：2004-11-01 00:00:00

abstract：BACKGROUND:Newborn screening for deficiency in the lysosomal enzymes that cause Fabry, Gaucher, Krabbe, Niemann-Pick A/B, and Pompe diseases is warranted because treatment for these syndromes is now available or anticipated in the near feature. We describe a multiplex screening method for all five lysosomal enzymes tha...

journal_title：Clinical chemistry

authors： Li Y,Scott CR,Chamoles NA,Ghavami A,Pinto BM,Turecek F,Gelb MH

更新日期：2004-10-01 00:00:00

abstract：BACKGROUND:Screening for heterozygous sequence changes in PCR products, also known as "mutation scanning", is an important tool for genetic research and clinical applications. Conventional methods require a separation step. METHODS:We evaluated the sensitivity and specificity of homogeneous scanning, using a saturatin...

journal_title：Clinical chemistry

authors： Reed GH,Wittwer CT

更新日期：2004-10-01 00:00:00

abstract：BACKGROUND:Recent use of Bacillus anthracis spores as a bioweapon has highlighted the need for a continuous monitoring system. Current monitoring systems rely on antibody-derived probes, which are not hardy enough to withstand long-term use under extreme conditions. We describe new, phage-derived probes that can be use...

journal_title：Clinical chemistry

authors： Brigati J,Williams DD,Sorokulova IB,Nanduri V,Chen IH,Turnbough CL Jr,Petrenko VA

更新日期：2004-10-01 00:00:00

abstract：BACKGROUND:C-Reactive protein (CRP) has been shown to be a strong predictor of coronary heart disease (CHD) and is being considered in cardiovascular disease risk assessment. The number of normolipidemic individuals who are eligible for evaluation of CRP in overall CHD risk assessment is not known. METHODS:We analyzed...

journal_title：Clinical chemistry

authors： Ajani UA,Ford ES,Mokdad AH

更新日期：2004-09-01 00:00:00

abstract：BACKGROUND:Mutations in the retina-specific ABC transporter (ABCA4) gene have been associated with several forms of macular degenerations. Because the high complexity of the molecular genotype makes scanning of the ABCA4 gene cumbersome, we describe here the first use of denaturing HPLC (DHPLC) to screen for ABCA4 muta...

journal_title：Clinical chemistry

authors： Stenirri S,Fermo I,Battistella S,Galbiati S,Soriani N,Paroni R,Manitto MP,Martina E,Brancato R,Allikmets R,Ferrari M,Cremonesi L

更新日期：2004-08-01 00:00:00

abstract：BACKGROUND:Lead is an environmental pollutant, and human exposure is assessed by monitoring lead concentrations in blood. Because the main source of environmental exposure has been the use of leaded gasoline, its phase-out has led to decreased lead concentrations in the general population. Therefore, validated analytic...

journal_title：Clinical chemistry

authors： Patriarca M,Castelli M,Corsetti F,Menditto A

更新日期：2004-08-01 00:00:00

abstract：BACKGROUND:The differential diagnosis of D-2-hydroxyglutaric aciduria (D-2-HGA), L-2-hydroxyglutaric aciduria (L-2-HGA), and the combined D/L-2-hydroxyglutaric aciduria (D/L-2-HGA) can be accomplished only by the measurement of the corresponding 2-hydroxyglutarate (2-HG). Available methods for the determination of D- a...

journal_title：Clinical chemistry

authors： Struys EA,Jansen EE,Verhoeven NM,Jakobs C

更新日期：2004-08-01 00:00:00

abstract：BACKGROUND:The sex differential in coronary heart disease (CHD) risk, which is not explained by male/female differences in lipid and lipoprotein concentrations, narrows with age. We examined whether this differential CHD risk might, in part, be attributable to the sizes of lipoprotein particles or concentrations of lip...

journal_title：Clinical chemistry

authors： Freedman DS,Otvos JD,Jeyarajah EJ,Shalaurova I,Cupples LA,Parise H,D'Agostino RB,Wilson PW,Schaefer EJ

更新日期：2004-07-01 00:00:00

abstract：INTRODUCTION:Pro or precursor forms of prostate-specific antigen (PSA) have emerged as potentially important diagnostic serum markers for prostate cancer detection. Immunoassays were developed to measure specific proPSA forms containing propeptides of 2, 4, and 7 amino acids [(-2)proPSA, (-4)proPSA, and (-7)proPSA, res...

journal_title：Clinical chemistry

authors： Mikolajczyk SD,Catalona WJ,Evans CL,Linton HJ,Millar LS,Marker KM,Katir D,Amirkhan A,Rittenhouse HG

更新日期：2004-06-01 00:00:00

abstract：BACKGROUND:Analysis of fetal DNA in maternal plasma has recently been introduced as a new method for noninvasive prenatal diagnosis, particularly for the analysis of fetal genetic traits, which are absent from the maternal genome, e.g., RHD or Y-chromosome-specific sequences. To date, the analysis of other fetal geneti...

journal_title：Clinical chemistry

authors： Li Y,Zimmermann B,Rusterholz C,Kang A,Holzgreve W,Hahn S

更新日期：2004-06-01 00:00:00

abstract：BACKGROUND:Specific gravity (SG) may perform as well as creatinine (CR) correction for adjusting urinary hormone concentrations, as well as offer some advantages. We compared the two methods and applied them to US and Bangladeshi specimens to evaluate their use in different populations. METHODS:Pearson correlations be...

journal_title：Clinical chemistry

authors： Miller RC,Brindle E,Holman DJ,Shofer J,Klein NA,Soules MR,O'Connor KA

更新日期：2004-05-01 00:00:00

abstract：BACKGROUND:Hemoglobin-based oxygen carriers (HBOCs) are being developed as potential substitutes for the oxygen-carrying functions of erythrocytes, but athletes may obtain and experiment with HBOCs as an illicit means of enhancing oxygen transport. An electrophoretic technique has been developed to screen for the prese...

journal_title：Clinical chemistry

authors： Varlet-Marie E,Ashenden M,Lasne F,Sicart MT,Marion B,de Ceaurriz J,Audran M

更新日期：2004-04-01 00:00:00

abstract：BACKGROUND:Understanding the distribution of C-reactive protein (CRP) concentration among adult women in the US helps to establish the magnitude of women at increased risk for cardiovascular disease. METHODS:We examined the distribution and correlates of CRP, using data from 2205 women >or=20 years of age from the Nat...

journal_title：Clinical chemistry

authors： Ford ES,Giles WH,Mokdad AH,Myers GL

更新日期：2004-03-01 00:00:00

abstract：BACKGROUND:Eph receptors and their ligands, the ephrins, represent a large class of cell-cell communication molecules with well-defined developmental functions. Their role in healthy adult tissues and in human disease is still largely unknown, although diverse roles in carcinogenesis have been postulated. METHODS:We e...

journal_title：Clinical chemistry

authors： Hafner C,Schmitz G,Meyer S,Bataille F,Hau P,Langmann T,Dietmaier W,Landthaler M,Vogt T

更新日期：2004-03-01 00:00:00

abstract：BACKGROUND:Nandrolone is one of the most abused anabolic steroids, and its use in doping is increasing, as revealed by numerous positive cases during recent years in various sports. Different authors have reported the possible natural production of nandrolone metabolites in humans, and some of these authors argued that...

journal_title：Clinical chemistry

authors： Baume N,Avois L,Schweizer C,Cardis C,Dvorak J,Cauderay M,Mangin P,Saugy M

更新日期：2004-02-01 00:00:00